General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress MEK1 Kinase Binding Assay, 10pl Kit. (96-0040E15CP0L)
The InCELL Hunter eXpress MEK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of MEK1 via detection of MEK1 levels. This product uses EFC technology. The cell line included overexpresses MEK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay, 10-plate Kit. (96-0046E1CP7L)
The InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HCK Kinase Binding Assay, 10pl Kit. (96-0041E15CP0L)
The InCELL Hunter eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cell line included overexpresses HCK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay, 2-plate Kit. (96-0009E3CP16M)
The InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2L1 target in an untagged form and Bax binding partner fused to ProLabel. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Rat IL-13 ASY
The R-PLEX Rat IL-13 Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures rat interleukin 13 (IL-13) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and shows minimal cross-reactivity The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker reagents and calibrator The assay takes 3 5 hours to complete and has a sensitivity of 0 3 pg/mL
Meso Scale Discovery R-PLEX Rat IL-4 ASY
The Meso Scale Discovery R-PLEX Rat IL-4 Assay is a multiplex immunoassay panel that allows simultaneous measurement of rat interleukin 4 (IL-4) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on MSD instrument platforms The R-PLEX Rat IL-4 Assay provides a reproducible and sensitive method for measuring rat IL-4 levels in research studies
Cayman Chemical GlutathIon Reductase asy K 96
GR is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized GSSG to GSH. This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. Cayman’s Glutathione Reductase Assay Kit measures GR activity by measuring the rate of NADPH oxidation. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the GR activity in the sample.
Cayman Chemical 2-bromo 17-EstradIol 5mg
A synthetic steroid and inhibitor of estrogen 2-hydroxylase; inhibits estrogen 2-hydroxylase in rat liver microsomes at 50 and 100 µM; prevents estrogen-induced inhibition of apomorphine-induced climbing behavior in mice at 5 mg/kg
Cayman Chemical Hydroxybutyrate Assay BuFR 1ea
A peptide derivative of a-MSH and a synthetic intermediate
Cayman Chemical cPLA2 asy Buffer 1ea
An SHP-2 inhibitor (IC50 E76K (IC50 0.17 UM) reduces the levels of phosphorylated ERK in a reporter assay using KYSE-520 cancer cells (IC90 0.012 UM) and inhibits proliferation of the same cells (IC50 0.32 UM) reduces tumor volume in KYSE-520 and NCI H358 mouse xenograft models at 30 or 100 mg/kg two times per day
Cayman Chemical LPO asy FTS Reagent 2 1ea
A medium-chain acyl CoA inhibitsbutyryl-CoAacetate-CoA transferase isolated from P. elsdenii at 200 UM levels are increased in primary fibroblasts derived from patients with MCAD deficiency in the presence of heptanoic acid blocks berberine-induced decreases in infarct volume and improvements in motor function in a mouse model of MCAO-induced ischemic stroke at 0.4 mg/kg per day